Normal view MARC view ISBD view

Cellular Pathology Technique.

By: Culling, C. F. A.
Contributor(s): Allison, R. T | Barr, W. T.
Material type: TextTextSeries: eBooks on Demand.Publisher: Cambridge : Elsevier Science, 2014Copyright date: ©1985Edition: 4th ed.Description: 1 online resource (651 pages).Content type: text Media type: computer Carrier type: online resourceISBN: 9781483192192.Subject(s): Cytodiagnosis -- Technique | Histochemistry -- Technique | Pathology, Cellular -- TechniqueGenre/Form: Electronic books.Additional physical formats: Print version:: Cellular Pathology TechniqueDDC classification: 616.0758 Online resources: Click here to view this ebook.
Contents:
Front Cover -- Cellular Pathology Technique -- Copyright Page -- Table of Contents -- Preface -- Acknowledgements -- Part I: Introduction -- Chapter 1. Cells and tissues -- The nucleus -- The cytoplasm -- Cell division -- Organization of cells into tissues -- Colloidal conception of tissue -- Chapter 2. Outline of methodology -- Preparation -- Demonstration -- Examination -- Part II: Fixation, processing and microtomy -- Chapter 3. Fixation -- Introduction -- Theoretical considerations of fixation -- Reagents employed as fixatives -- Fixatives -- Micro-anatomical fixatives -- Cytological fixatives -- Spray fixatives -- Histochemical fixatives -- Post chroming and secondary fixation -- Vapour fixation -- Freeze-drying -- Freeze substitution -- References -- Chapter 4. Processing -- Factors affecting processing -- Paraffin-wax processing and embedding -- Instrumentation for paraffin-wax processing -- Rapid processing in paraffin wax -- Ester wax -- Water-soluble waxes -- Gelatin embedding -- Celloidin and LVN (Low Viscosity Nitrocellulose) -- The dry celloidin method -- Double embedding -- Resin embedding -- Critical-point drying -- References -- Chapter 5. Microtomy -- Microtomes -- Microtomy -- References -- Part III: Staining and impregnation -- Chapter 6. Theoretical -- Staining -- Metachromasia -- Leucocompounds -- Fluorescent dyes -- Effects of fixation -- Progressive and regressive staining. Differentiation -- Mordants -- Accentuators -- Direct colour production -- Controls in biological staining -- Classification of dyes -- Impregnation -- References -- Chapter 7. Staining procedure -- Preparation of stains -- Solvents -- Basic staining and mounting procedures -- Mountants -- Ringing media -- References -- Part IV: Demonstration methods -- Chapter 8. Haematoxylin and its counterstains -- Haematoxylins -- Counterstains.
Specialized haematoxylins -- References -- Chapter 9. Connective tissue -- Methods -- References -- Chapter 10. Nucleic acids -- Deoxyribonucleic acid (DNA) -- Ribonucleic acid (RNA) -- Demonstration of nucleic acids -- Routine staining -- Techniques for deoxyribonucleic acid -- Techniques for ribonucleic acid -- Enzyme extraction of nucleic acids -- Chemical extraction of nucleic acids -- References -- Chapter 11. Proteins -- Introduction -- Chemistry -- Identification of proteins -- General methods for protein -- Methods for specific groups of amino acids -- Carboxyl groups -- Blocking methods for proteins -- References -- Chapter 12. Carbohydrates -- Classification of naturally occurring polysaccharides (Figure 12.1) -- Fixation of carbohydrates -- Identification of carbohydrates -- Methods for sialic acids -- References -- Chapter 13. Lipids -- Definitions -- General chemistry of lipids -- Classification -- Fixation of lipids -- Identification of lipids -- References -- Chapter 14. Endogenous pigments -- Haem pigments -- Tyrosine pigments -- Lipid pigments -- References -- Chapter 15. Deposits -- Artefact deposits -- Exogenous pigments, minerals and inorganic constituents -- References -- Chapter 16. Enzymes -- Preservation -- Methods of demonstration -- The use of controls -- Enzyme techniques -- References -- Chapter 17. Micro-organisms -- Bacteria -- Spirochaetes -- Fungi -- Protozoa -- References -- Part V: Immunocytochemistry -- Chapter 18. Immunofluorescence -- Conjugates -- Techniques -- Preparation of material -- Staining technique -- Applications -- References -- Chapter 19. Immunoenzyme technique -- Types of method (see Figure 19.1) -- Notes on technique -- General procedure -- References -- Part VI: Tissues of special interest -- Chapter 20. The nervous system -- Anatomy -- Composition.
Processing and section cutting of the central nervous system -- Methods of demonstration -- Methods for rapid diagnosis -- References -- Chapter 21. Hard tissue -- Bone -- Specimens -- Microtomy -- Teeth -- References -- Chapter 22. Miscellaneous biopsies -- Kidney -- Skin -- Eye -- Pancreas -- Alimentary canal -- Lymph nodes -- Bone marrow -- References -- Chapter 23. Muscle -- Demonstration -- The skeletal muscle biopsy -- Muscle biopsy for study of innervation -- Cardiac biopsies -- References -- Part VII: Cells and cell products of special interest -- Chapter 24. Miscellaneous cells -- Paneth cells -- Eosinophils -- Mast cells -- Plasma cells -- Corpora amylacea -- Alcoholic hyaline (Mallory bodies) -- Juxtaglomerular cells -- References -- Chapter 25. Amyloid and fibrin -- Amyloid -- Fibrin -- References -- Chapter 26. Endocrine cells -- Introduction -- Demonstration -- References -- Chapter 27. Cytology technique -- Technique -- Miscellaneous -- Staining methods -- Chromosome techniques -- Sex chromatin -- References -- Part VIII: Special techniques -- Chapter 28. Quantitative methods -- Preparation of tissues -- Morphology -- Measurement of section thickness -- Reaction -- References -- Chapter 29. Autoradiography -- References -- Chapter 30. Micro-incineration -- Reference -- Chapter 31. Museum technique -- Preparation, colour maintenance, fixation and storage of specimens -- Mounting of museum specimens -- Special methods -- References -- Chapter 32. Photomicrography -- Introduction -- Preparation of sections -- Optics -- Illumination -- Black and white photomicrography -- Colour photomicrography -- Colour films -- Reciprocity law failure -- References -- Part IX: Microscopy -- Chapter 33. Microscopy -- General microscopy -- Lens -- Component parts of a compound microscope -- Optical components -- Mechanical components.
Setting up the microscope -- Cleaning and maintenance -- Magnification -- Micrometry -- Darkground microscopy -- Objectives and condensers -- Setting up the darkground microscope -- Fluorescence microscopy -- Fluorescence -- Equipment -- Microscopic preparations -- Autofluorescence (primary fluorescence) -- Fluorescence techniques -- Polarizing microscopy -- Theoretical aspects -- Equipment -- Setting up the microscope -- Differential interference contrast microscopy -- Interference microscopy -- References -- Part X: Electron microscopy -- Chapter 34. The electron microscope -- Transmission electron microscopy (TEM) -- Scanning electron microscope (SEM) -- Scanning transmission electron microscope (STEM) -- Analytical systems -- References -- Index.
Summary: Cellular Pathology Technique aims to maintain the twin objectives of producing a comprehensive bench book and a text for students that will take the Special Examination in Cellular Pathology of the Institute of Medical Laboratory Sciences. The organization of this fourth edition has been reshaped. Some sections were expanded such as those about the theory of staining, and new chapters were added dealing with immunolocalization, the endocrine system, and quantification. This book is organized into 10 parts. The introductory part provides basic information on cells and tissues and outlines the methodology in cellular pathology techniques. This is followed by chapters that deal with various aspects of cellular pathology including tissues, cells and cell products of special interests, electron microscopy, and immunocytochemistry. This book will be of interest to students of cellular pathology and those in the medical profession.
Tags from this library: No tags from this library for this title. Log in to add tags.
Item type Current location Call number URL Status Date due Barcode
Electronic Book UT Tyler Online
Online
RB43 -- .C855 1985 (Browse shelf) http://ebookcentral.proquest.com/lib/uttyler/detail.action?docID=1879871 Available EBC1879871

Front Cover -- Cellular Pathology Technique -- Copyright Page -- Table of Contents -- Preface -- Acknowledgements -- Part I: Introduction -- Chapter 1. Cells and tissues -- The nucleus -- The cytoplasm -- Cell division -- Organization of cells into tissues -- Colloidal conception of tissue -- Chapter 2. Outline of methodology -- Preparation -- Demonstration -- Examination -- Part II: Fixation, processing and microtomy -- Chapter 3. Fixation -- Introduction -- Theoretical considerations of fixation -- Reagents employed as fixatives -- Fixatives -- Micro-anatomical fixatives -- Cytological fixatives -- Spray fixatives -- Histochemical fixatives -- Post chroming and secondary fixation -- Vapour fixation -- Freeze-drying -- Freeze substitution -- References -- Chapter 4. Processing -- Factors affecting processing -- Paraffin-wax processing and embedding -- Instrumentation for paraffin-wax processing -- Rapid processing in paraffin wax -- Ester wax -- Water-soluble waxes -- Gelatin embedding -- Celloidin and LVN (Low Viscosity Nitrocellulose) -- The dry celloidin method -- Double embedding -- Resin embedding -- Critical-point drying -- References -- Chapter 5. Microtomy -- Microtomes -- Microtomy -- References -- Part III: Staining and impregnation -- Chapter 6. Theoretical -- Staining -- Metachromasia -- Leucocompounds -- Fluorescent dyes -- Effects of fixation -- Progressive and regressive staining. Differentiation -- Mordants -- Accentuators -- Direct colour production -- Controls in biological staining -- Classification of dyes -- Impregnation -- References -- Chapter 7. Staining procedure -- Preparation of stains -- Solvents -- Basic staining and mounting procedures -- Mountants -- Ringing media -- References -- Part IV: Demonstration methods -- Chapter 8. Haematoxylin and its counterstains -- Haematoxylins -- Counterstains.

Specialized haematoxylins -- References -- Chapter 9. Connective tissue -- Methods -- References -- Chapter 10. Nucleic acids -- Deoxyribonucleic acid (DNA) -- Ribonucleic acid (RNA) -- Demonstration of nucleic acids -- Routine staining -- Techniques for deoxyribonucleic acid -- Techniques for ribonucleic acid -- Enzyme extraction of nucleic acids -- Chemical extraction of nucleic acids -- References -- Chapter 11. Proteins -- Introduction -- Chemistry -- Identification of proteins -- General methods for protein -- Methods for specific groups of amino acids -- Carboxyl groups -- Blocking methods for proteins -- References -- Chapter 12. Carbohydrates -- Classification of naturally occurring polysaccharides (Figure 12.1) -- Fixation of carbohydrates -- Identification of carbohydrates -- Methods for sialic acids -- References -- Chapter 13. Lipids -- Definitions -- General chemistry of lipids -- Classification -- Fixation of lipids -- Identification of lipids -- References -- Chapter 14. Endogenous pigments -- Haem pigments -- Tyrosine pigments -- Lipid pigments -- References -- Chapter 15. Deposits -- Artefact deposits -- Exogenous pigments, minerals and inorganic constituents -- References -- Chapter 16. Enzymes -- Preservation -- Methods of demonstration -- The use of controls -- Enzyme techniques -- References -- Chapter 17. Micro-organisms -- Bacteria -- Spirochaetes -- Fungi -- Protozoa -- References -- Part V: Immunocytochemistry -- Chapter 18. Immunofluorescence -- Conjugates -- Techniques -- Preparation of material -- Staining technique -- Applications -- References -- Chapter 19. Immunoenzyme technique -- Types of method (see Figure 19.1) -- Notes on technique -- General procedure -- References -- Part VI: Tissues of special interest -- Chapter 20. The nervous system -- Anatomy -- Composition.

Processing and section cutting of the central nervous system -- Methods of demonstration -- Methods for rapid diagnosis -- References -- Chapter 21. Hard tissue -- Bone -- Specimens -- Microtomy -- Teeth -- References -- Chapter 22. Miscellaneous biopsies -- Kidney -- Skin -- Eye -- Pancreas -- Alimentary canal -- Lymph nodes -- Bone marrow -- References -- Chapter 23. Muscle -- Demonstration -- The skeletal muscle biopsy -- Muscle biopsy for study of innervation -- Cardiac biopsies -- References -- Part VII: Cells and cell products of special interest -- Chapter 24. Miscellaneous cells -- Paneth cells -- Eosinophils -- Mast cells -- Plasma cells -- Corpora amylacea -- Alcoholic hyaline (Mallory bodies) -- Juxtaglomerular cells -- References -- Chapter 25. Amyloid and fibrin -- Amyloid -- Fibrin -- References -- Chapter 26. Endocrine cells -- Introduction -- Demonstration -- References -- Chapter 27. Cytology technique -- Technique -- Miscellaneous -- Staining methods -- Chromosome techniques -- Sex chromatin -- References -- Part VIII: Special techniques -- Chapter 28. Quantitative methods -- Preparation of tissues -- Morphology -- Measurement of section thickness -- Reaction -- References -- Chapter 29. Autoradiography -- References -- Chapter 30. Micro-incineration -- Reference -- Chapter 31. Museum technique -- Preparation, colour maintenance, fixation and storage of specimens -- Mounting of museum specimens -- Special methods -- References -- Chapter 32. Photomicrography -- Introduction -- Preparation of sections -- Optics -- Illumination -- Black and white photomicrography -- Colour photomicrography -- Colour films -- Reciprocity law failure -- References -- Part IX: Microscopy -- Chapter 33. Microscopy -- General microscopy -- Lens -- Component parts of a compound microscope -- Optical components -- Mechanical components.

Setting up the microscope -- Cleaning and maintenance -- Magnification -- Micrometry -- Darkground microscopy -- Objectives and condensers -- Setting up the darkground microscope -- Fluorescence microscopy -- Fluorescence -- Equipment -- Microscopic preparations -- Autofluorescence (primary fluorescence) -- Fluorescence techniques -- Polarizing microscopy -- Theoretical aspects -- Equipment -- Setting up the microscope -- Differential interference contrast microscopy -- Interference microscopy -- References -- Part X: Electron microscopy -- Chapter 34. The electron microscope -- Transmission electron microscopy (TEM) -- Scanning electron microscope (SEM) -- Scanning transmission electron microscope (STEM) -- Analytical systems -- References -- Index.

Cellular Pathology Technique aims to maintain the twin objectives of producing a comprehensive bench book and a text for students that will take the Special Examination in Cellular Pathology of the Institute of Medical Laboratory Sciences. The organization of this fourth edition has been reshaped. Some sections were expanded such as those about the theory of staining, and new chapters were added dealing with immunolocalization, the endocrine system, and quantification. This book is organized into 10 parts. The introductory part provides basic information on cells and tissues and outlines the methodology in cellular pathology techniques. This is followed by chapters that deal with various aspects of cellular pathology including tissues, cells and cell products of special interests, electron microscopy, and immunocytochemistry. This book will be of interest to students of cellular pathology and those in the medical profession.

Description based on publisher supplied metadata and other sources.

There are no comments for this item.

Log in to your account to post a comment.